Paradoxical Effect of Nonalcoholic Red Wine Polyphenol Extract, Provinols™, in the Regulation of Cyclooxygenases in Vessels from Zucker Fatty Rats (fa/fa)

Figure 6

Provinols modulated the expression of COX isoforms and NF-κB in vessels from obese ZF rats. Western blots showing the expression of cyclooxygenase- (COX-) 1 (a, b) and COX-2 (c, d) in the aorta and SMA from ZF rats treated or not with Provinols. NF-κB expression in the rat aorta was assessed by confocal imaging (e) and western blot analysis (f). In (c) is shown NF-κB expression in SMA by western blot analysis. Quantification of immunoblots’ signal was done by densitometric analysis; β-actin loading control was included. Data are representative of four separate blots, and the densitometry values are expressed in arbitrary units (A.U.) as mean ± SEM. ; versus ZF rats.

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