Progression of Hepatic Adenoma to Carcinoma in Ogg1 Mutant Mice Induced by Phenobarbital

Figure 3

(a) Results of H&E staining and immunohistochemical evaluation of several hepatocarcinogenesis biomarkers (A–H) and p-Nrf2 (I, J) in the livers, HCC (A, C, E, G, and I), and HCA (B, D, F, H, and J) of Ogg1^−/− and Ogg1^+/+ mice maintained on 500 ppm PB for 78 weeks. Note that the nuclear expression of p-Nrf2 in PB-treated Ogg1^+/+ mice HCAs (arrow), but no staining in Ogg1^−/− HCCs. (b) Immunohistochemistry for 8-OHdG (A–D) and PCNA (E–H) in the surrounding liver tissue of Ogg1^−/− and Ogg1^+/+ mice treated with PB for 78 weeks. Note the high expression of 8-OHdG (A) and PCNA (E) in the livers of Ogg1^−/− mice treated with PB (arrows).

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