Progression of Hepatic Adenoma to Carcinoma in Ogg1 Mutant Mice Induced by Phenobarbital

Figure 4

Representative blots for phospho-Nrf2 (p-Nrf2 (S40)) (a) and total Nrf2 (b) of Ogg1^−/− and Ogg1^+/+ PB-treated and control mice are presented. PB induced elevation of total Nrf2 protein levels in the surrounding liver tissue and liver tumors of Ogg1 null and mostly in the wild-type mice. However, p-Nrf2 was observed only in the livers and HCAs of wild-type animals, indicating that sufficient Nrf2 activation occurs only in PB-administered Ogg1^+/+ mouse livers. HCC: hepatocellular carcinoma; HCA: hepatocellular adenoma; S: surrounding liver tissue; C: untreated control liver.

(a)

(b)