Molecular Hydrogen Effectively Heals Alkali-Injured Cornea via Suppression of Oxidative Stress
The immunohistochemical detection of cytokeratins K3/K12 and the expression of genes for cytokeratins K3 (e) and K12 (f) determined by real-time PCR in injured corneas treated with H2 dissolved in PBS (H2) or with PBS free of H2 (buffer) examined on day 20 after the injury. The expression of K3/K12 was high in injured reepithelialized corneas treated with H2 (c), arrow points to the epithelium, whereas in injured corneas treated with buffer, where corneas were poorly reepithelialized (arrows), the expressions of K3/K12 were low or absent (a, b) compared to the control cornea (d). Scale bars: 50 μm. In graphs the values with asterisks represent statistically significant () difference from injured buffer treated corneas.
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