Molecular Hydrogen Effectively Heals Alkali-Injured Cornea via Suppression of Oxidative Stress
The immunohistochemical detection of iNOS and IL-1β and the expression of genes for IL-1β (g) determined by real-time PCR in injured corneas treated with H2 dissolved in PBS (H2) or with PBS free of H2 (buffer) examined on day 20 after the injury. The expressions of iNOS (a) and IL-1β (b) were high in buffer treated injured corneas, arrows, whereas in H2 treated injured corneas the expressions of iNOS (c) and IL-1β (d) were low or completely absent, similarly to in control cornea for iNOS (e) or IL-1β (f). Scale bars: 50 μm. In graph the values with asterisks represent statistically significant () difference from injured buffer treated corneas.
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