Oleic Acid and Hydroxytyrosol Inhibit Cholesterol and Fatty Acid Synthesis in C6 Glioma Cells

Figure 2

Modulation of cholesterol and fatty acid syntheses by oleic acid and/or hydroxytyrosol. After an initial 48 h plating, C6 glioma cells, growing in serum-rich medium, were incubated for 4 h with 25 μM OA and/or 25 μM HTyr. During the last hour of incubation, labelled acetate was added and its incorporation into cholesterol (a) and fatty acids (b) was followed. Data, nmol [1-¹⁴C]acetate/h/mg protein, are expressed as % of control and are means ± S.D. of six independent experiments. In each experiment, determinations were carried out in triplicate. In control cells, rates of cholesterol and fatty acid synthesis were 1.43 ± 0.07 and 8.67 ± 0.49 nmol [1-¹⁴C]acetate inc/h/mg protein, respectively. Samples bearing different letters differ significantly (). None: no addition to the cells; OA: oleic acid; HTyr: hydroxytyrosol.

(a)

(b)