Oleic Acid and Hydroxytyrosol Inhibit Cholesterol and Fatty Acid Synthesis in C6 Glioma Cells

Table 1

Effect of OA and HTyr and their combination on [1-¹⁴C]acetate incorporation into various lipid fractions in C6 cells

Added effectors	None	OA	HTyr	OA + HTyr
Polar lipids
CL + PE	2151 ± 151^a	1377 ± 85^b	1742 ± 121^c	1119 ± 75^d
PC	15,908 ± 875^a	5596 ± 308^b	12,781 ± 703^c	3487 ± 192^d
SM	741 ± 67^a	463 ± 37^b	640 ± 37^a	270 ± 19^c
PS + PI	3781 ± 246^a	1439 ± 101^b	2533 ± 165^c	962 ± 65^d
Neutral lipids
MG	289 ± 20^a	231 ± 11^b,c	252 ± 11^b	214 ± 7^c
DG	711 ± 136^a	587 ± 24^a	623 ± 27^a	562 ± 35^a
Cholesterol	2155 ± 194^a	1724 ± 155^b	1896 ± 170^a,b	1509 ± 136^b
Unesterified fatty acids	239 ± 14^a	200 ± 12^a,b	187 ± 11^b	123 ± 8^b
TG	1123 ± 73^a	1048 ± 68^a,b	999 ± 65^a,b	921 ± 60^b
CE	896 ± 63^a	659 ± 46^b,c	725 ± 47^a,b	587 ± 23^c

C6 cells were incubated with 25 μM oleic acid (OA) and/or 25 μM hydroxytyrosol (HTyr) for 4 h, and labeled acetate was added 1 h before ending the incubation. Total lipids were extracted. Phospholipids and neutral lipids were resolved by TLC, and the radioactivity associated with the different lipid fractions was counted. CL: cardiolipin; PE: phosphatidylethanolamine; PC: phosphatidylcholine; SM: sphingomyelin; PS: phosphatidylserine; PI: phosphatidylinositol; MG: monoglycerides; DG: diglycerides; TG: triglycerides; CE: cholesterol esters. Values are expressed as cpm/mg protein ± SD, . Within the same group, samples bearing different letters differ significantly ().