Differential Alterations of the Mitochondrial Morphology and Respiratory Chain Complexes during Postnatal Development of the Mouse Lung

Figure 10

Electron micrographs showing immunogold labelling for mitochondrial proteins in ultrathin sections of ciliated cells in newborn (NB), P15, and adult (AL) animals. Lung tissue processed for immunoelectron microscopy was incubated with gold-labelled secondary antibody particles and thereafter contrasted with uranyl acetate and lead citrate prior to analysis by transmission electron microscopy. (a–i) Immunogold labelling in mitochondria of ciliated cells for (a–c) complex III (UQCR2), (d, e) complex IV (COX1), and (g, h) complex V (ATP6E). Bars represent 0.5 μm.