Differential Alterations of the Mitochondrial Morphology and Respiratory Chain Complexes during Postnatal Development of the Mouse Lung

Table 2

List of primers used in this study for qRT-PCR (the annealing temp was 60°C).

Full name	Gene target	Sense primer (5′–3′) 20–23 mers	Antisense primer (5′–3′) 20–24 mers	Size of product
Mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 1	mt-Nd1	GCTTTACGAGCCGTAGCCCA	GGGTCAGGCTGGCAGAAGTAA	147
Mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 2	mt-Nd2	CCTCCTGGCCATCGTACTCA	GAATGGGGCGAGGCCTAGTT	124
Mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 3	mt-Nd3	TAGTTGCATTCTGACTCCCCCA	GAGAATGGTAGACGTGCAGAGC	100
Mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 4	mt-Nd4	CGCCTACTCCTCAGTTAGCCA	TGATGTGAGGCCATGTGCGA	112
Mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 4L	mt-Nd4l	AGCTCCATACCAATCCCCATCAC	GGACGTAATCTGTTCCGTACGTGT	109
Mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 5	mt-Nd5	GGCCCTACACCAGTTTCAGC	AGGGCTCCGAGGCAAAGTAT	134
Mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 6	mt-Nd6	CTTGATGGTTTGGGAGATTGG	ACCCGCAAACAAAGATCACC	138
Succinate dehydrogenase complex subunit D	Sdhd	GCTCGAGCTCTCCTACTCC	GCTTGGTGACAGGTGAATGT	117
Mitochondrially encoded cytochrome b	mt-Cytb	TCCTTCATGTCGGACGAGGC	AATGCTGTGGCTATGACTGCG	100
Mitochondrially encoded cytochrome c oxidase I	mt-Co1, Cox1	TCAACATGAAACCCCCAGCCA	GCGGCTAGCACTGGTAGTGA	100
Mitochondrially encoded cytochrome c oxidase II	mt-Co2, Cox2	ACCTGGTGAACTACGACTGCT	TCCTAGGGAGGGGACTGCTC	121
Mitochondrially encoded cytochrome c oxidase III	mt-Co3, Cox3	CCAAGGCCACCACACTCCTA	GGTCAGCAGCCTCCTAGATCA	150
Mitochondrially encoded ATP synthase 6	mt-Atp6	AGCTCACTTGCCCACTTCCT	AAGCCGGACTGCTAATGCCA	114
Mitochondrially encoded 12S RNA	mt-Rnr1	ACACCTTGCCTAGCCACACC	GTGGCTGGCACGAAATTTACCA	112
Mitochondrially encoded 16S RNA	mt-Rnr2	ACACCGGAATGCCTAAAGGA	ATACCGCGGCCGTTAAACTT	148
Transcription factor B1, mitochondrial	Tfb1m	GGCTGAGAGACTTGTAGCCACT	AGGTGCACCACTCCTACATCAA	150
Transcription factor B2, mitochondrial	Tfb2m	TTTGGCAAGTGGCCTGTGAC	ACTGATTCCCCGTGCTTTGACT	109
Mitochondrial transcription factor A	Tfam	GCCCGGCAGAGACGGTTAAA	GCCGAATCATCCTTTGCCTCC	137
Polymerase (RNA) mitochondrial	Polrmt	ACAACACCGTGATGCTTGGC	GAACATCCTGGTCCCTGCGT	150
Polymerase (DNA) gamma 2, accessory subunit	Polg2	CTGGTTGCGTCATCGGCTTC	TGCTTCCCTTGCGTCCCAAT	101
Glyceraldehyde 3-phosphate dehydrogenase	GAPDH	TGGCAAAGTGGAGATTGTTGCC	AAGATGGTGATGGGCTTCCCG	156