Erratum to “Resveratrol Attenuates Copper-Induced Senescence by Improving Cellular Proteostasis”

Figure 1

Reduced sirtuin 1 (Sirt1) expression in copper sulfate stress-induced premature senescent (CuSO₄-SIPS) fibroblasts is restored by the addition of resveratrol. (a-b) WI-38 fibroblasts were incubated with 350 µM CuSO₄ (or Na₂SO₄, for controls) for 24 h. Then, the media were changed and cells were allowed to recover for an additional 72 h in the presence of 5 or 10 µM resveratrol (or 0.1% dimethyl sulfoxide [DMSO] for controls). After this recovery period, fibroblasts were processed for different assays. (a) Sirt1 transcript levels were assessed by quantitative (q)PCR and plotted with the assumption that control mRNA levels equaled 1. TATA box binding protein (TBP) was the selected housekeeping gene. (b) Sirt1 relative protein content was determined by western blot, using Ponceau S staining to normalize protein loading. Depicted blots are representative and densitometric quantification is plotted with the assumption that control cells in the absence of resveratrol represent a relative protein level of 1. Data are presented as means ± SEM of at least three independent experiments. and , when compared to control cells in the absence of resveratrol; , relative to CuSO₄-treated cells without resveratrol.

(a)

(b)