Erratum to “Resveratrol Attenuates Copper-Induced Senescence by Improving Cellular Proteostasis”

Figure 6

CuSO₄-induced accumulation of carbonylated and polyubiquitinated proteins is reduced by resveratrol via induction of lysosomal autophagy. (a) Protein carbonyl content and (b) polyubiquitinated (poly-Ub) proteins were evaluated in fibroblasts exposed to the indicated conditions by western blot. Representative blots are depicted and densitometric quantification was normalized by assigning a value of 1 to the control cells in the absence of resveratrol. Ponceau S staining was used to account for differences in protein loading. (c) Lysosomal autophagy was studied by measuring the conversion of LC3-I to LC3-II, a critical step for autophagosome formation, and quantification of P62, a ubiquitin-binding protein that targets Ub substrates to autophagosomes. The LC3-II/LC3-I ratio and relative levels of P62 were evaluated via densitometric quantification and plotted with the assumption that control cells without resveratrol represent a value of 1. Data are presented as means ± SEM of at least three independent experiments. ; ; ; and , for the comparisons between the indicated groups.

(a)

(b)

(c)