4-Hydroxynonenal Contributes to Angiogenesis through a Redox-Dependent Sphingolipid Pathway: Prevention by Hydralazine Derivatives

Figure 5

Effect of hydralazine and BVH on the angiogenic signaling of 4-HNE. (a) Chemical structures of hydralazine (Hdz), bisvanillin (BV), and bisvanillyl-hydralazone (BVH). (b) Effect of Hdz and BVH (10 µM each), on ROS generated by HMEC-1 after 30 min of contact with 4-HNE (0.5 µM). (c) Effect of Hdz and BVH (10 µM each), on SK1 activation by 4-HNE, measured after 90 min incubation. (d) Effect of Hdz, BVH, and BV (10 µM each) on tube formation elicited by 4-HNE (0.5 µM). These data are means ± SEM of 4 separate experiments. ; ns: not significant.

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