Low Glucose Concentrations Induce a Similar Inflammatory Response in Monocytes from Type 2 Diabetic Patients and Healthy Subjects
Table 2
Paired differences comparing IL-1β (a) and IL-10 (b) levels after monocyte incubation at different glucose concentrations, with (LPS+) or without (LPS−) lipopolysaccharide stimulation.
(a)
IL-1β paired differences (pg/mL)
Controls ()
Controls ()
Diabetic patients ()
Diabetic patients ()
LPS−
LPS+
LPS−
LPS+
G5 − G2.5
−56 ± 17∗∗
−94 ± 28∗∗
−79 ± 32∗
−171 ± 57∗∗
G20 − G5
3 ± 8
−6 ± 10
−8 ± 8
0.5 ± 9.6
(b)
IL-10 paired differences (pg/mL)
Controls ()
Controls ()
Diabetic patients ()
Diabetic patients ()
LPS−
LPS+
LPS−
LPS+
G5 − G2.5
−3 ± 1
−5 ± 6
−3 ± 2
29 ± 20
G20 − G5
0 ± 1
−21 ± 4∗∗
−2 ± 1
−36 ± 9∗∗
G2.5: glucose 2.5 mmol/L; G5: glucose 5.0 mmol/L; G20: glucose 20 mmol/L. Data are mean ± SEM. Paired comparisons were obtained by changing one experimental variable at a time. Negative values indicate a higher IL-1β (a) and IL-10 (b) concentration in the latter condition considered. ,. Student’s t-test for paired data indicating a significant change between the paired experimental conditions stated in the first column.
