Arsenite Effects on Mitochondrial Bioenergetics in Human and Mouse Primary Hepatocytes Follow a Nonlinear Dose Response

Figure 2

Arsenite effect on mitochondrial bioenergetics in primary hepatocytes. Mitochondrial bioenergetic profile of (a) mouse and (b) human primary hepatocytes in response to increasing concentration of arsenite. Primary hepatocytes in the presence of glucose were exposed sequentially to oligomycin (OLIGO), carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP), and rotenone/antimycin A (ROT/ANTI A) as described in methods. Nonmitochondrial respiration after the final addition of ROT/ANTI A was subtracted from the other values. BR, basal respiration; OLIGO, oligomycin-sensitive or ATP-linked respiration; FCCP, maximal respiration in the presence of FCCP. Tables to the right of (a) and (b) provide the statistical analysis of the bioenergetic profile. Results representative of 5 independent experiments. Each independent experiment was run using an independent mouse and an independent human liver (representing 5 different mouse and 5 different human livers). Bioenergetic profile data presented as mean ± SD.

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