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Figure 2: Decreased fission and fusion protein levels in SAD cells. (a) Representative Western blot analysis and quantification of DLP1 in healthy and SAD fibroblasts under basal conditions. (b) Representative confocal microscopy images showing TOMM20 in green and DLP1 in red in basal conditions. (c) Quantification of the amount of DLP1 in mitochondria per cell in the images represented in (b). (d) Quantification of mitochondrial surface measured by TOMM20 label per cell in the images represented in (b). (e)–(g) Representative Western blot analysis and quantification of MFN2/mitofusin 2 (f) and STOML2 (g) in healthy and SAD fibroblasts under basal conditions. Graphs show means and standard deviations of the healthy/AD age- and sex-matched couple samples: AG11020/AG05810 and AG11362/AG05809; AG07310/AG06869; AG05813/AG06263; AG07803/AG06262 (a)–(e) and AG11020/AG05810; AG11362/AG05809; and AG07310/AG06869 (g). Scale bar: 40 μm. .