Ginkgolide B Suppresses TLR4-Mediated Inflammatory Response by Inhibiting the Phosphorylation of JAK2/STAT3 and p38 MAPK in High Glucose-Treated HUVECs

Figure 4

Synergistic inhibitory effect of ginkgolide B on the expression of TLR4 and PAFR in PAFR siRNA- and TLR4 siRNA-treated HUVECs. HUVECs were transfected by siRNA against PAFR or siRNA against TLR4 for 48 h to knock down PAFR or TLR4. The HUVECs were treated with 0.6 mg/ml ginkgolide B for 1 h, and glucose was then added for another 8 h. TLR4 and PFAR expression was analyzed by Western blot. (a) TLR4 expression in PAFR siRNA-transfected cells. (b) PAFR expression in TLR4 siRNA-transfected cells. Data for the density analysis were obtained from three independent experiments. ^&, significant difference between negative siRNA-transfected cells (negative control (NC)) and PAFR siRNA-transfected cells or TLR4 siRNA-transfected cells; ^#, significant difference between nonhigh glucose-treated cells and high glucose-treated cells; , significant difference between non-ginkgolide B-treated cells and ginkgolide B-treated cells.

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