Ginkgolide B Suppresses TLR4-Mediated Inflammatory Response by Inhibiting the Phosphorylation of JAK2/STAT3 and p38 MAPK in High Glucose-Treated HUVECs

Figure 5

Ginkgolide B inhibits the phosphorylation of JAK2 and STAT3 in high glucose-treated HUVECs. The cells were treated with various concentrations of ginkgolide B for 1 h, and glucose was added for another 8 h. Phosphorylated JAK2 and STAT3 were analyzed by Western blot. (a, b) Ginkgolide B inhibited JAK2 and STAT3 phosphorylation that was induced by high glucose. (c) Phosphorylated STAT3 expression was determined by immunofluorescence. (d) CLI-095 inhibited STAT3 phosphorylation that was induced by LPS. (e) The combination of ginkgolide B and CLI-095 synergistically inhibited STAT3 phosphorylation that was induced by high glucose. ^#, significant difference between nonhigh glucose-treated cells and high glucose-treated cells; , significant difference between non-ginkgolide B-treated cells and ginkgolide B-treated cells.

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