Pterostilbene 4′-β-Glucoside Protects against DSS-Induced Colitis via Induction of Tristetraprolin
4-PG induces TTP mRNA and protein in RAW264.7 cells. (a) Chemical structure of pterostilbene (PTER) and pterostilbene 4′-glucoside (4-PG). (b–d) RAW264.7 cells were treated at various concentrations of 4-PG (0, 1, 5, 10, and 20 μM) and PTER (10 μM) for 12 h. The mRNA and protein expression of TTP were determined by RT-PCR (b), real-time PCR (c) and Western blot (d), respectively. (e–g) RAW264.7 cells were treated with 4-PG (10 μM) and PTER (10 μM) in indicated time points (0, 2, 4, 8, and 12 h). The mRNA and protein expression of TTP were analyzed by RT-PCR (e), real-time PCR (f), and Western blot (g), respectively. (h) To compare the effect of 4-PG and PTER on TTP protein expression, RAW264.7 cells were treated with 4-PG (10 μM) and PTER (10 μM) for 12 h. The protein level of TTP was measured by Western blot assay. ImageJ software was used for densitometry analysis. GAPDH and β-actin were used as internal controls. Data are expressed as means ± SE, . , , versus cells without treatment.