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Oxidative Medicine and Cellular Longevity
Volume 2017, Article ID 9456163, 10 pages
https://doi.org/10.1155/2017/9456163
Research Article

Protective Effects of Dinitrosyl Iron Complexes under Oxidative Stress in the Heart

Russian Cardiology Research and Production Complex, Ministry of Healthcare of the Russian Federation, 3rd Cherepkovskaya St., Building 15a, Moscow 121552, Russia

Correspondence should be addressed to Vladimir P. Shirinsky; moc.liamg@yksnirihs

Received 6 January 2017; Accepted 27 February 2017; Published 21 March 2017

Academic Editor: Andrey J. Serra

Copyright © 2017 Valery I. Kapelko et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Nitric oxide can successfully compete with oxygen for sites of electron-transport chain in conditions of myocardial hypoxia. These features may prevent excessive oxidative stress occurring in cardiomyocytes during sudden hypoxia-reoxygenation. Aim. To study the action of the potent stable NO donor dinitrosyl iron complex with glutathione (Oxacom®) on the recovery of myocardial contractile function and Ca2+ transients in cardiomyocytes during hypoxia-reoxygenation. Results. The isolated rat hearts were subjected to 30 min hypoxia followed by 30 min reoxygenation. The presence of 30 nM Oxacom in hypoxic perfusate reduced myocardial contracture and improved recovery of left ventricular developed pressure partly due to elimination of cardiac arrhythmias. The same Oxacom concentration limited reactive oxygen species generation in hypoxic cardiomyocytes and increased the viability of isolated cardiomyocytes during hypoxia from 12 to 52% and after reoxygenation from 0 to 40%. Oxacom prevented hypoxia-induced elevation of diastolic Ca2+ level and eliminated Ca2+ transport alterations manifested by slow Ca2+ removal from the sarcoplasm and delay in cardiomyocyte relaxation. Conclusion. The potent stable NO donor preserved cardiomyocyte integrity and improved functional recovery at hypoxia-reoxygenation both in the isolated heart and in cardiomyocytes mainly due to preservation of Ca2+ transport. Oxacom demonstrates potential for cardioprotection during hypoxia-reoxygenation.