Protective Effects of Dinitrosyl Iron Complexes under Oxidative Stress in the Heart

Figure 4

Effects of Oxacom on Ca²⁺ transients in isolated adult rat cardiomyocytes subjected to hypoxia-reoxygenation. Cardiomyocytes loaded with Fluo-4 were preincubated with 30 nM Oxacom for 30 min and electrostimulated by ten rectangular pulses at 1 Hz under conditions of normoxia, after 2 min of hypoxia produced by exchanging regular perfusion buffer saturated with carbogen (95% O₂, 5% CO₂) for hypoxic buffer saturated with 95% N₂ and 5% CO₂ and then after 15 min of reoxygenation. Typical recordings are shown. The legend for ordinate on the left applies to all graphs.