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Oxidative Medicine and Cellular Longevity
Volume 2017 (2017), Article ID 9460653, 24 pages
https://doi.org/10.1155/2017/9460653
Research Article

Antihypertensive Effects of Roselle-Olive Combination in L-NAME-Induced Hypertensive Rats

1Pharmacology Department, National Research Centre, Giza, Egypt
2Chemistry Department, College of Science, King Khalid University, Abha, Saudi Arabia
3Biochemistry Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
4Pathology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
5Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt

Correspondence should be addressed to Rehab F. Abdel-Rahman

Received 6 June 2017; Revised 13 August 2017; Accepted 28 August 2017; Published 22 October 2017

Academic Editor: Neelam Khaper

Copyright © 2017 Rehab F. Abdel-Rahman et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study aimed to evaluate the antihypertensive efficacy of a new combination therapy of Hibiscus sabdariffa and Olea europaea extracts (2 : 1; Roselle-Olive), using N(G)-nitro-L-arginine-methyl ester- (L-NAME-) induced hypertensive model. Rats received L-NAME (50 mg/kg/day, orally) for 4 weeks. Concurrent treatment with Roselle-Olive (500, 250, and 125 mg/kg/day for 4 weeks) resulted in a dose-dependent decrease in both systolic and diastolic blood pressure, reversed the L-NAME-induced suppression in serum nitric oxide (NO), and improved liver and kidney markers, lipid profile, and oxidative status. Furthermore, Roselle-Olive significantly lowered the elevated angiotensin-converting enzyme activity (ACE) and showed a marked genoprotective effect against oxidative DNA damage in hypertensive rats. Roselle-Olive ameliorated kidney and heart lesions and reduced aortic media thickness. Real-time PCR and immunohistochemistry showed an enhanced endothelial nitric oxide synthase (eNOS) gene and protein expression in both heart and kidney of Roselle-Olive-treated rats. To conclude, our data revealed that Roselle-Olive is an effective combination in which H. sabdariffa and O. europaea synergistically act to control hypertension. These effects are likely to be mediated by antioxidant and genoprotective actions, ACE inhibition, and eNOS upregulation by Roselle-Olive constituents. These findings provide evidences that Roselle-Olive combination affords efficient antihypertensive effect with a broad end-organ protective influence.