Low-Dose Ionizing Radiation Affects Mesenchymal Stem Cells via Extracellular Oxidized Cell-Free DNA: A Possible Mediator of Bystander Effect and Adaptive Response

Table 3

Dependence of the changes in the levels of antiapoptotic genes BCL2, BCL2A1 (Bfl-1/A1), BCL2L1 (BCL-X), BIRC2 (c-IAP1), and BIRC3 (c-IAP2) and proapoptotic BAX expression in irradiated and exposed to cfDNAox, cfDNAoxR, and gDNA in MSCs on the time after exposure (RT-PCR). mRNA level—average expression of genes in treated MSCs compared to control (for three biological replicates). Reference gene—TBP. , nonparametric U test (qRT-PCR).

Gene	Changes in the expression levels, arb.un.
Treatment	Time	10 cGy	cfDNAox	cfDNAoxR	gDNA
BCL2	30 min	2.6 ± 0.3	2.3 ± 0.2	2.4 ± 0.2	1.1 ± 0.2
	2 h	4.1 ± 0.4	3.1 ± 0.2	3.3 ± 0.3	1.0 ± 0.2
	24 h	3.9 ± 0.3	2.9 ± 0.2	2.9 ± 0.3	1.3 ± 0.3
	48 h	2.8 ± 0.3	3.1 ± 0.3	2.6 ± 0.2	1.8 ± 0.3
BCL2A1 (Bfl-1/A1)	30 min	1.6 ± 0.2	2.4 ± 0.2	2.5 ± 0.3	1.0 ± 0.2
	2 h	2.1 ± 0.2	2.2 ± 0.1	2.4 ± 0.2	1.1 ± 0.2
	24 h	2.1 ± 0.3	2.8 ± 0.3	2.3 ± 0.2	1.3 ± 0.2
	48 h	1.8 ± 0.3	1.6 ± 0.2	2.2 ± 0.2	1.7 ± 0.3
BCL2L1 (BCL-X)	30 min	1.4 ± 0.2	1.6 ± 0.1	1.6 ± 0.1	1.1 ± 0.2
	2 h	1.9 ± 0.3	1.5 ± 0.2	1.7 ± 0.2	1.2 ± 0.3
	24 h	2.3 ± 0.2	2.7 ± 0.3	2.4 ± 0.2	1.1 ± 0.3
	48 h	2.8 ± 0.3	2.4 ± 0.3	1.9 ± 0.2	1.6 ± 0.2
BIRC2 (c-IAP1)	30 min	2.4 ± 0.2	1.4 ± 0.2	1.8 ± 0.2	1.0 ± 0.3
	2 h	2.6 ± 0.2	2.1 ± 0.2	2.3 ± 0.3	1.0 ± 0.2
	24 h	2.6 ± 0.3	2.7 ± 0.3	2.6 ± 0.2	1.6 ± 0.3
	48 h	2.4 ± 0.2	2.4 ± 0.3	2.5 ± 0.2	2.5 ± 0.3
BIRC3 (c-IAP2)	30 min	2.2 ± 0.1	1.2 ± 0.2	1.2 ± 0.2	1.0 ± 0.2
	2 h	2.5 ± 0.2	1.9 ± 0.2	1.8 ± 0.2	1.2 ± 0.3
	24 h	2.5 ± 0.2	2.6 ± 0.3	2.5 ± 0.3	1.6 ± 0.3
	48 h	2.0 ± 0.3	2.5 ± 0.4	2.5 ± 0.4	2.0 ± 0.4
BAX	30 min	1.6 ± 0.2	1.5 ± 0.2	1.4 ± 0.2	1.0 ± 0.2
	2 h	0.8 ± 0.2	0.8 ± 0.2	0.9 ± 0.2	1.1 ± 0.3
	24 h	0.8 ± 0.2	0.9 ± 0.2	1.1 ± 0.2	0.9 ± 0.2