Cytoprotective Effects of Cell-Permeable Bifunctional Antioxidant Enzyme, GST-TAT-SOD, against Cisplatin-Induced Cell Damage

Figure 2

Biochemical estimations of L-02 cells treated with cisplatin. (a) MDA level, (b) T-AOC, (c) SOD activity, and (d) GST activity. Cells were treated with protein (2000 U/mL) for 3 h followed by 0.7 μg/mL cisplatin. After treatment, the cells were further cultured for 24 h at 37°C. Then, the cells were lysed and centrifuged, and the antioxidant activities and protein contents in the supernatants were determined subsequently. The bars indicate the means ± SD (). The CDDP group was compared with the control group (, ). The pretreated group was compared with the CDDP group (^∗P < 0.05).

(a)

(b)

(c)

(d)