The Citrus Flavanone Naringenin Protects Myocardial Cells against Age-Associated Damage

Figure 5

Effect of Nar on the levels of released estradiol and estrogen-regulated gene mRNA expression. (a) Each bar represents the mean ± SEM of three replicates from three independent experiments. The measured estradiol levels were within the reference range [24]. Specific calibration curves and appropriate positive/negative controls were carried out during the assay. and versus the control; versus H₂O₂-treated cells. (b) ER β and (c) VDR mRNA expression as indicators of estrogenic activity in the senescent H9c2 cells. Each bar represents the mean ± SEM of three replicates from three independent experiments. , , and versus the control; and versus H₂O₂-treated cells.

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