The Citrus Flavanone Naringenin Protects Myocardial Cells against Age-Associated Damage

Table 1

Nucleotide sequences, annealing temperature, and product size of the primers utilized in real-time RT-PCR experiments.

Gene	Primer nucleotide sequences	Annealing temperature (°C)	Product size (base pairs)
p16	FOR 5′- CCGAGAGGAAGGCGAACTC -3′	66.3	76
	REV 5′- GCTGCCCTGGCTAGTCTATCTG -3′	66.2
p21	FOR 5′- GAGCAAAGTATGCCGTCGTC -3′	64.7	127
	REV 5′- CTCAGTGGCGAAGTCAAAGTTC -3′	65.0
ER β	FOR 5′- CTACAGAGAGATGGTCAAAAGTGGA -3′	64.4	218
	REV 5′- GGGCAAGGAGACAGAAAGTAAGT -3′	63.6
VDR	FOR 5′- GTGACTTTGACCGGAACGTG -3′	65.6	280
	REV 5′- ATCATCTCCCTCTTACGCTG -3′	60.8