Activation of NLRP3 Inflammasome by Advanced Glycation End Products Promotes Pancreatic Islet Damage

Figure 6

AGE-induced mice pancreatic NLRP3 inflammasome activation through ROS/TXNIP pathway. C57BL/6J mice were injected intraperitoneally with BSA, AGEs, or AGEs plus the antioxidant NAC (40 mM in drinking water) for 6 weeks. (a) Representative photomicrographs of mice pancreatic sections stained with DHE. Scale bar = 50 μm. Histogram represents quantitative analysis of superoxide anion generation in each experimental group. per group. Representative blots and Western blot analyses of NOX2 (b), TXNIP (c), and NLRP3 (d) protein expression in mice pancreas tissue lysates. per group. (e) Representative blot and quantification of immunoprecipitation (IP) with TXNIP and blotting (IB) with NLRP3 showed higher association of TXNIP with NLRP3 ( per group), which was associated with increased caspase-1 activity ((f), per group) and enhanced cleaved IL-1β expression ((g, h, i), per group). Values are expressed as mean ± SD, ^# versus BSA group. versus AGEs group. MFI: mean fluorescence intensity.

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