Mitochondrial Respiration in Intact Peripheral Blood Mononuclear Cells and Sirtuin 3 Activity in Patients with Movement Disorders

Table 3

Comparison of mitochondrial respiration and sirtuin 3 (SIRT3) expression/activity in a subgroup of patients with movement disorders with substantia nigra hyperechogenicity and in controls. Results are presented as mean ± standard deviation (ROUTINE and ROX) and median (interquartile range for LEAK, ETS, and SIRT3). All outcomes of mitochondrial respiration are measured in pmol O2/s10⁶ cells. SIRT3 protein expression is represented as μg/mg protein. SIRT3 activity is represented as U/μg protein.

	Controls (C)	Substantia nigra hyperechogenicity present (SNH+); (59%)	Substantia nigra hyperechogenicity absent (SNH-); (41%)	(SNH+) versus C	(SNH−) versus C	(SNH+) versus (SNH−)
ROUTINE; (pmol O2/s10^6 cells)	19.3 ± 4.4	14.1 ± 3.6	15.9 ± 4.9	0.007	0.1332	0.3768
LEAK; (pmol O2/s10^6 cells)	6.02; 5.37–6.78	3.4; 2.1–5.2	4.4; 3.6–5.5	0.0203	0.0822	0.6603
ETS; (pmol O2/s10^6 cells)	13.34; 11.22–17.39	12.1; 9.9–15.2	12.2; 9.3–14.3	0.1947	0.3465	0.9167
ROX; (pmol O2/s10^6 cells)	5.7 ± 1.6	4.1 ± 2.0	4.1 ± 2.5	0.0801	0.1337	0.9484
SIRT3 expression; (μg/mg protein)	63.9; 20.5–115.4	35.5; 24.0–47.8	42.7; 19.4–60.1	0.9606	0.6666	0.9606
SIRT3 activity; (U/μg protein)	181.17; 60.9–344.6	90.1; 37.3–141.2	66.6; 34.7–137.3	0.6570	0.0364	0.6570