Spontaneous Production of Glutathione-Conjugated Forms of 1,2-Dichloropropane: Comparative Study on Metabolic Activation Processes of Dihaloalkanes Associated with Occupational Cholangiocarcinoma

Figure 3

Relative levels of halogenated hydrocarbon metabolites in the presence of GSTT1. (a) #P1, (b) #P2, (c) #M1, and (d) #M3. Reaction mixtures containing 1% (v/v) (a and b) 1,2-DCP or (c and d) DCM in the presence or absence of GSTT1 protein (50 μg/mL) were incubated at 37 °C for 60 min, followed by semiquantification of the levels of each metabolite in the resulting solution. Data were normalized against those acquired in the absence of GSTT1 conditions. Data were expressed as means ± SD, . Statistical analyses for significant differences were performed using Student’s t-test. . N.S.: not significantly different among groups.

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