Figure 2: The activation of the NLRP3 inflammasome and expression of caspase-1 and IL-1β were increased in diabetic rats after MI/R insult. NLRP3 and caspase-1 expression in heart tissues were examined by immunohistochemistry (a). The expression of NLRP3 (b), ASC (c), procaspase-1 and caspase-1 (d), and IL-1β (e) were analyzed by Western blot. Data are expressed as the mean ± SD. . versus sham; # versus Ctrl + sham; and ## versus Ctrl + I/R.