Figure 4: HG and H/R stimulate caspase-1 activation and pyroptosis in cultured H9C2 cells. The expression of caspase-1 (p10) was analyzed by Western blot (a). Cell viability was detected by CCK-8 assay (b). The LDH release was detected by activity assays (c). Caspase-1 activity was determined by caspase-1 activity assay (d). IL-1β content was measured by using ELISA kits (e). Calcein-AM/PI staining was used for the cell death assay; green indicates living cells, and red indicates dead cells (f). Data are expressed as the mean ± SD. per group. and versus LG; and && versus HG; ## versus LG + H/R; and ◆◆ versus HG + H/R.