Treatment Effects of Ischemic Stroke by Berberine, Baicalin, and Jasminoidin from Huang-Lian-Jie-Du-Decoction (HLJDD) Explored by an Integrated Metabolomics Approach

Figure 10

Effects of HLJDD and its three principal components on inflammatory responses. (a) Boxplots for the gene expression levels of COX2, iNOS, TNF-α, IL-1β, IL-2, and IL-6 in brains by qRT-PCR in each group (). The line drawn at the bottom of each box, in the box, and at the top of the box represented the 1st, 2nd, and 3rd quartiles, respectively. The whiskers extended to ±1.5 times the interquartile range (from the 1st to 3rd quartile). All the data obtained were expressed as mean ± standard deviation (SD). ^## and ^###, MCAO group versus sham group; , , and , drug-treated groups versus MCAO group. (b) The protein level of COX-2 and iNOS in brains treated with HLJDD and its three principals (). (c) The protein level of cytosolic p-IKBα, p-IKK, and p-p65 and nuclear p-p65 in brains treated with HLJDD and its three principals (). (d) The protein level of p65 in brain tissues of rats treated with HD, Ber, Bai, and Jas determined by immunohistochemical staining. Results of quantitative analysis values are expressed as mean ± SD (). ^## and ^###, MCAO group versus sham group; , , and , drug-treated groups versus MCAO group.

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