Thioredoxin-1 Protects Bone Marrow-Derived Mesenchymal Stromal Cells from Hyperoxia-Induced Injury In Vitro

Figure 4

Effect of Trx-1 on cell apoptosis in BMSCs. Cells were exposed to hyperoxia for 0, 12, 24, and 48 hours and were stained with annexin V-PE/7-ADD before flow cytometry analysis. (a) Dot plots of flow cytometry analysis. Intensity of 7-ADD staining (y-axis) was plotted versus annexin V intensity (x-axis). Numbers indicate percent in each region. (b) The graph shows the percentage of apoptosis as defined by annexin V⁺. The results are representative of 3 independent experiments. (c) Caspase 3 activity. Caspase 3 activity was measured by the caspase 3 activity kit. Bar graphs represent the relative expression of caspase 3 activity calculated from each group. The results are representative of 3 independent experiments. , compared with the BMSCs-p group or BMSCs. BMSCs: intact BMSCs; BMSCs-p: empty lentivirus-engineered BMSCs; BMSCs-Trx-1: Trx-1-engineered BMSCs.

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