Vialinin A, an Edible Mushroom-Derived p-Terphenyl Antioxidant, Prevents VEGF-Induced Neovascularization In Vitro and In Vivo

Figure 3

Effect of vialinin A on HUVEC tube formation in vitro. Growth-arrested HUVECs were pretreated with different concentrations of vialinin A (1 μM, 2 μM, and 5 μM) followed by treatment with VEGF (10 ng/mL) for 24 h. In vitro Matrigel-based angiogenesis assay was performed using an angiogenesis kit from EMD Millipore following the manufacturer’s instructions. (a) Images showing the effect of different concentrations of vialinin A (0 μM, 1 μM, 2 μM, and 5 μM) on growth factor-induced tube formation. Representative images from three independent experiments are shown. Magnification 4x. Scale bar = 1000 μm. (b) Bars showing quantification of tube length performed by measuring individual tube length in different areas. Values are the mean ± SD. and when compared to untreated control.

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