Changes of KEAP1/NRF2 and IKB/NF-κB Expression Levels Induced by Cell-Free DNA in Different Cell Types
The exposure to cfDNA reduces apoptosis frequency in MSC. (a) Annexin V fluorescence is plotted along the y-axis, and PI fluorescence is along the x-axis. From top to bottom: controls, after 3 hours of exposure to p(rDNA) in a concentration of 50 ng/ml. R area contains cells with low PI and high annexin V fluorescence, that is, apoptotic cells. (b) The percentage frequency of cells belonging to the framed R fraction, that is, with the signs of apoptosis. Significantly different from the control (). gDNA: genomic DNA; gDNAoxy1 and gDNAoxy2: oxidized genomic DNA with two different levels of oxidation; p(rDNA): ribosomal repeat; p(rDNA)oxy: oxidized ribosomal repeat.