Changes of KEAP1/NRF2 and IKB/NF-κB Expression Levels Induced by Cell-Free DNA in Different Cell Types
Expression levels of BCL2 family genes BCL2, BCL2A1, BCL2L1, BIRC2 (c-IAP1), and BIRC3 (c-IAP2) after an exposure of HUVEC (a) and MCF7 (b) to oxidized and nonoxidized GC-rich fragments of model cfDNA (50 ng/ml, see the exposure duration in the figure). Averaged values of three independent tests on HUVEC cultures derived from three different donors, and SD values are shown. As an internal standard gene, ТВР gene was used. The horizontal green lines show the mean gene expression level of intact endothelial cells (1 ± 0.2 arb.un.). Values are significantly different from the control (, Mann–Whitney U test). gDNA: genomic DNA; gDNAoxy: oxidized genomic DNA; p(rDNA): ribosomal repeat.