Effects of Post Ischemia-Reperfusion Treatment with Trimetazidine on Renal Injury in Rats: Insights on Delayed Renal Fibrosis Progression
Effects of postischemia-reperfusion (I/R) treatment with TMZ on renal tubular apoptotic cell death. Representative histology of renal tubules after TUNEL assay (a) and a percentage of apoptotic cells (apoptotic cells/total cells) (b). Compared to the sham groups, I/R injury resulted in significant apoptotic cell death in the IRC groups at 24 h and 5 d after I/R injury. TMZ significantly attenuated apoptotic cell death compared to the IRC groups at 24 h and 5 d after I/R injury. At 8 w after I/R injury, TUNEL assay of renal tissues demonstrated almost full recovery of histopathologic findings in the IRC and the TMZ-treated groups, without intergroup differences. TMZ = trimetazidine; TUNEL = terminal deoxynucleotidyl transferase-mediated uridine triphosphate nick end labeling. Sham = rats not underwent I/R; IRC = rats underwent ischemia (45 min)-reperfusion (24 h, 5 d, and 8 w); TMZS = rats treated with TMZ (3 mg/kg) upon reperfusion; TMZD = rats treated with TMZ (3 mg/kg) once daily for 5 d or 8 w starting upon reperfusion. versus sham, versus IRC.