Effects of Post Ischemia-Reperfusion Treatment with Trimetazidine on Renal Injury in Rats: Insights on Delayed Renal Fibrosis Progression
Effects of postischemia-reperfusion (I/R) treatment with TMZ on the expressions of Bcl-2 and Bax. Western blot analysis (a) and densitometric analysis (b, c) of the Western blot for Bcl-2 and Bax at 24 h, 5 d, and 8 w after I/R injury, respectively. Bcl-2 level was significantly decreased, and Bax level was significantly increased in the IRC groups compared to that in the sham groups at 24 h and 5 d after I/R injury. Bcl-2 level was significantly higher, and Bax level was significantly lower in the TMZ-treated groups than that in the IRC groups. At 8 w after renal I/R injury, Bcl-2 and Bax levels were significantly greater in the IRC and the TMZ-treated groups than those in the sham groups, without any intergroup differences between the IRC and the TMZ-treated groups. Sham = rats not underwent I/R; IRC = rats underwent ischemia (45 min)-reperfusion (24 h, 5 d, and 8 w); TMZS = rats treated with TMZ (3 mg/kg) upon reperfusion; TMZD = rats treated with TMZ (3 mg/kg) once daily for 5 d or 8 w starting upon reperfusion. versus sham, versus IRC.