Effects of Post Ischemia-Reperfusion Treatment with Trimetazidine on Renal Injury in Rats: Insights on Delayed Renal Fibrosis Progression
Effects of postischemia-reperfusion (I/R) treatment with TMZ on the expressions of MMPs and TIMPs. Western blot analysis (a) and densitometric analysis (b–e) of the Western blot for MMP-2, -9 and TIMP-1, -2 at 5 d, and 8 w after I/R injury, respectively. Post I/R treatment with TMZ reduced the degree of I/R-induced increases in MMP-2 and MMP-9 levels and further increased the levels of TIMP-1 and TIMP-2 at 5 d after renal I/R injury compared to the IRC group. At 8 w after I/R injury, MMP levels in the TMZ-treated groups were similar to those of the IRC groups, which were all significantly greater than those of the sham groups. TIMP levels in the TMZ-treated groups became also comparable to those of the IRC groups, which were significantly greater than those of the sham groups. TMZ = trimetazidine. Sham = rats not underwent I/R; IRC = rats underwent ischemia (45 min)-reperfusion (5 d or 8 w); TMZS = rats treated with TMZ (3 mg/kg) upon reperfusion; TMZD = rats treated with TMZ (3 mg/kg) once daily for 5 d or 8 w starting upon reperfusion. versus sham, versus IRC.