Gallic Acid-L-Leucine Conjugate Protects Mice against LPS-Induced Inflammation and Sepsis via Correcting Proinflammatory Lipid Mediator Profiles and Oxidative Stress

Figure 3

Effects of GAL on the chemotactic potential of macrophages. (a) Effect of GAL on the chemotactic potential of RAW264.7 cells. After 24 h treatment with GAL and LPS, the cell culture medium was recovered and assayed for the chemotactic potential in Transwell assay. The cells that migrated through Transwell membrane were sequentially fixed, stained, and imaged under a microscope. Representative images were shown. Macrophage migration was quantified by measuring the absorbance of the cell lysates. The results represent the means ± SD of three separate experiments. (GAL-LPS versus LPS alone). (b) Effect of GAL on the chemotactic potential of primary peritoneal macrophages. The chemotactic potential of primary peritoneal macrophages was analyzed as described.

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