The Critical Role of IL-10 in the Antineuroinflammatory and Antioxidative Effects of Rheum tanguticum on Activated Microglia

Figure 10

Possible components of Rt responsible for the production of IL-10 in microglia. (a) The change in the mRNA of IL-10 in MG6 cells 24 h after treatment with components of Rt, including chrisophanol, physcion, β-sitosterol, emodin, aloe-emodin, (+)-catechin, and piceatannol. The results show the relative mRNA expression of IL-10 after treatment with aloe-emodin (10 μM), (+)-catechin (30 μM), and piceatannol (100 μM) with the minimum effective doses, which were determined after treatment with three different doses (10, 30, and 100 μM) for 24 h. On the other hand, chrisophanol, physcion, β-sitosterol, and emodin with the concentration of 100 μM were applied for 24 h. The results represent the mean ± SEM of three independent experiments. The asterisks indicate a statistically significant difference from the indicated value (, ). (b) The secretion of IL-10 in the medium of MG6 cells and primary cultured microglia after treatment with aloe-emodin, (+)-catechin, and piceatannol. The results show the mean IL-10 secreted in the culture medium after treatment with aloe-emodin (10 μM for 48 h) and (+)-catechin (30 μM for 48 h) with the minimum effective doses for the most effective experimental time points. The most effective experimental time points were determined after examination of three different experimental time points (24, 48 and 72 h). On the other hand, piceatannol with 100 μM was applied for 72 h. The results represent the mean ± SEM of three independent experiments. The asterisks indicate a statistically significant difference from the value in untreated cells ().

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