Losartan Ameliorates Calcium Oxalate-Induced Elevation of Stone-Related Proteins in Renal Tubular Cells by Inhibiting NADPH Oxidase and Oxidative Stress

Figure 3

ROS and NADPH oxidase were upregulated under stimulation of CaOx crystals. (a) NRK-52E cells were induced by COM (1 mM) for 6 h with or without apocynin preincubation, and then intracellular ROS was detected by flow cytometry. Expression of Nox2 and Nox4 was upregulated in CaOx-induced NRK-52E cells (b) and rat kidneys in the hyperoxaluria group (c), as determined by Western blotting analysis, and the alteration could be reversed by apocynin or losartan administration. (d) COM (1 mM) reduced cellular SOD and CAT activities and increased MDA and 8-OHdG expression in NRK-52E cells which was reversed by apocynin administration. The data are expressed as mean ± SEM. compared with the Ang II group or control group and compared with the COM + Ang II group or hyperoxaluria group.

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