Losartan Ameliorates Calcium Oxalate-Induced Elevation of Stone-Related Proteins in Renal Tubular Cells by Inhibiting NADPH Oxidase and Oxidative Stress

Figure 5

CaOx induced ROS generation and overproduction of stone-related proteins was activated via Ang II/AT1R. (a) The mRNA level of AT1R in NRK-52E cells transfected with AT1R siRNA-1, siRNA-2, or siRNA-3 compared with the negative control group (NC group) (scrambled siRNA transfected) and the mock group (transfection reagent treated only). compared with the NC group and compared with the mock group. (b) The protein level of AT1R was downregulated in the COM + Ang II + AT1R siRNA-3 group compared with the COM + Ang II group. (c) The ROS generation was decreased in the COM + Ang II + AT1R siRNA-3 group compared with the COM + Ang II group. The expression of NADPH oxidase subunits (Nox2 and Nox4) (d), NF-κB subunits (p50 and p65) (e), and stone-related proteins (OPN, CD44, and MCP-1) (f) in the COM + Ang II and COM + Ang II + AT1R siRNA-3 groups was detected by Western blotting in NRK-52E cells. The data are expressed as mean ± SEM. compared with the Ang II group and compared with the COM + Ang II group. compared with the Ang II group and the COM + Ang II group.

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