Resveratrol Inhibits ROS-Promoted Activation and Glycolysis of Pancreatic Stellate Cells via Suppression of miR-21

Figure 1

Resveratrol (RSV) inhibits hydrogen peroxide (H₂O₂)-promoted pancreatic stellate cell (PSC) activation, invasion, and migration. (a, b) PSCs were treated with increasing doses of RSV (0, 12.5, 25, 50, 100, and 200 μM) for 24 h, 48 h, and 72 h or H₂O₂ (0, 12.5, 25, 37.5, 50, 62.5, 75, 87.5, 100, 150, 200, and 300 μM) for 24 h and then subjected to MTT assays to evaluate cell viability. (c–f) PSCs were treated with 50 μM RSV or 10 mM N-acetyl-L-cysteine (NAC), a ROS scavenger, for 24 h prior to the 24 h incubation with 50 μM H₂O₂. Protein levels of α-SMA were analyzed using Western blot and immunofluorescence staining of α-SMA. The red signal represents α-SMA staining, and nuclear DNA staining by DAPI is shown in blue (magnification, ×200; scale bar: 50 μm). (g, h) PSCs were treated in groups as indicated, and migratory ability was determined by wound healing assays. The relative distance moved by the leading edge marked by black lines was measured 24 h after wounding with a sterile pipette tip (magnification, ×100; scale bar: 100 μm). (i, j) PSCs were treated in groups as indicated, and invasive ability was determined by Matrigel invasion assays (magnification, ×200; scale bar: 50 μm). Column: mean; bar: SD; compared with the control group; compared with the H₂O₂ group.

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