Resveratrol Inhibits ROS-Promoted Activation and Glycolysis of Pancreatic Stellate Cells via Suppression of miR-21

Figure 6

RSV inhibits pancreatic cancer cell invasion and migration through suppression of ROS/miR-21 in PSCs. (a, b) PSCs were treated in groups as indicated and then cocultured with Panc-1 cells using Transwell chambers. The invasive ability of Panc-1 cells was determined by Matrigel invasion assays (magnification, ×200; scale bar: 50 μm). (c, d) PSCs were treated in groups as indicated and then cocultured with Panc-1 cells using Millicell hanging cell culture inserts. The migratory ability of Panc-1 cells was determined by wound healing assays. The relative distance moved by the leading edge marked by black lines was measured 24 h after wounding with a sterile pipette tip (magnification, ×100; scale bar: 100 μm). Column: mean; bar: SD; compared with the control group; compared with the PSC group; compared with the PSC (RSV) group.

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