Upregulation of SIRT1 by Kartogenin Enhances Antioxidant Functions and Promotes Osteogenesis in Human Mesenchymal Stem Cells

Figure 2

The effect of KGN on the expression and activity of superoxide dismutases. (a–b) The mRNA levels of SOD1 (a) and SOD2 (b) were measured using real-time RT-PCR. Values are the of four independent experiments () in real-time RT-PCR experiments. (c) Treatment with KGN at 10⁻⁷ M and 10⁻⁶ M increased the activity of SOD in BM-MSCs. Values are the of four independent experiments () in SOD activity experiments. (d) The increase in protein levels of SOD1 and SOD2 in KGN-treated BM-MSCs were confirmed using Western blot assays. (e–f) Quantification of protein levels of SOD1 (e) and SOD2 (f). Values are the of three independent experiments () in Western blot assays. Statistically significant differences are indicated by where between the indicated groups and where versus the CTRL group.

(a)

(b)

(c)

(d)

(e)

(f)