Upregulation of SIRT1 by Kartogenin Enhances Antioxidant Functions and Promotes Osteogenesis in Human Mesenchymal Stem Cells

Figure 3

The effect of KGN on the levels of CAT and GPX1. (a–b) The mRNA levels of CAT and GPX1 (b) were measured using real-time RT-PCR. Values are the of four independent experiments () in real-time RT-PCR experiments. (c) Treatment with KGN showed no effect on the activity of CAT. Values are the of four independent experiments () in CAT activity experiments. (d) The protein levels of CAT and GPX1 in KGN-treated BM-MSCs were determined using Western blot assays. (e–f) Quantification of protein levels of SOD1 (e) and SOD2 (f). Values are the of three independent experiments () in Western blot assays. Statistically significant differences are indicated by where between the indicated groups and where versus the CTRL group.

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