Intracellular and Intercellular Signalling Mechanisms following DNA Damage Are Modulated By PINK1
PINK1 loss of function enhances cellular sensitivity to DNA damage induced by BLM. (a) Schematic design of the study of DNA damage induced by genotoxic agents. The cells were exposed to genotoxic treatment (BLM for 1 h or X-rays) and incubated with fresh medium. At 24 h, cells were analyzed for the direct effects. Treatment of MEFs (b, d) and SH-SY5Y (c, e) with the DNA-damaging agent BLM at the indicated concentrations induces increased accumulation of MN (b, c) and γH2AX/53BP1 foci (d, e) as DNA damage endpoint indicators. PINK1-KO MEFs and PINK1-KD SH-SY5Y cells present higher sensitivity in the accumulation of both types of DNA damages with MEF cells being more chemosensitive than SH-SY5Y. Each data point represents the mean ± SEM of at least three independent experiments. Statistical analysis is performed with two-way ANOVA with multiple comparisons, and values are indicated in the figure. and .
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