Intracellular and Intercellular Signalling Mechanisms following DNA Damage Are Modulated By PINK1

Figure 4

PINK1 role in the transcriptional modulation of some DNA damage/repair factors following genotoxic stress induced by BLM. MEF cells from PINK1-KO and WT control have been treated with BLM at 40 μg/mL for one hour, and the cells were harvested 24 hours later for analysis of changes in transcription factors. DNA damage and repair (a, b) as well as signalling molecules (c, d) were investigated. PINK1 loss of function enhances stress signalling (b, d) while additional genotoxic stress appears to affect only one of the tested stress factors, DDIT4 (c). The data is reported as fold change versus WT untreated control. Each data point represents the mean ± SEM of at least three independent experiments. Statistical analysis was performed with two-way ANOVA with multiple comparisons, and values are indicated in the figure.

(a)

(b)

(c)

(d)