Effects of Low-Frequency Electromagnetic Field on Oxidative Stress in Selected Structures of the Central Nervous System

Table 3

Concentrations of oxidative stress biomarkers: malondialdehyde (MDA) and total oxidant status (TOS); activity of antioxidant enzymes: superoxide dismutase (SOD) and isoenzymes (SOD-Mn, SOD-CuZn), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione S-transferase (GST); and concentration of nonenzymatic antioxidants—total antioxidant capacity (TAC) in the brainstem homogenates from rats exposed to low-frequency electromagnetic field (group A) and sham-exposed rats (control group) (group C) with results of ANOVA for all examined groups (A, C, M, and A + M) and post hoc NIR test for two selected groups: A and C.

	Group A	Group C	ANOVA results
Parameter			; value	value of NIR test
MDA concentration (μmol/g protein)	1.93 ± 0.46	2.60 ± 0.38	3.12; 0.020	0.568
TOS concentration (μmol/g protein)	4.04 ± 1.25	3.19 ± 1.36	1.14; 0.349	0.217
SOD activity (NU/mg protein)	120.63 ± 5.58	144.45 ± 9.87	16.81; <0.001	<0.001
SOD-Mn activity (NU/mg protein)	63.79 ± 4.43	75.84 ± 6.37	14.76; <0.001	<0.01
SOD-CuZn activity (NU/mg protein)	56.84 ± 4.05	68.61 ± 8.48	17.26; <0.001	<0.01
CAT activity (IU/g protein)	11.64 ± 1.40	12.81 ± 1.10	14.26; <0.01	0.932
GPx activity (IU/g protein)	1.14 ± 0.31	1.38 ± 0.17	1.72; 0.181	0.103
GR activity (IU/g protein)	21.68 ± 1.55	28.95 ± 1.24	38.32; <0.001	<0.001
GST activity (IU/g protein)	3.77 ± 0.25	5.52 ± 0.54	34.71; <0.001	<0.001
TAC concentration (mmol/g protein)	0.12 ± 0.02	0.11 ± 0.04	0.57; 0.636	0.304