Implication of the PI3K/Akt/mTOR Pathway in the Process of Incompetent Valves in Patients with Chronic Venous Insufficiency and the Relationship with Aging

Figure 6

(a) Significant levels of mRNA for HIF-2α quantified by RT-qPCR in R. Results were normalized to that of the reference gene GAPDH and are provided in arbitrary units. NR = no reflux; R = reflux. . (b) Distribution of the percentage of patients with positive protein expression in HIF-2α NR and R patients and by age; n = number of patients. (c) A: Protein expression images of HIF-2α in cell nucleus (arrow) of patients (640x). B: Protein expression images of HIF-2α in capillary (arrow) of adventitia tunica in patients (640x). Red coloration indicates the specific precipitate that correlates with the expression of the said protein.

(a)

(b)

(c)